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| Registros recuperados: 11 | |
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| Cuvertino-Santoni,Jorge; Montenegro,Gloria. |
| Here, we present the current state of knowledge on the diversity of Chilean bryophytes (mosses, liverworts, and hornworts), the secondary metabolites present in these plants, and the biological activity of extracts from populations of Chilean species. Our goal is to establish the potential of these plants as a source of useful molecules for pharmaceutical and agricultural industries, thus promoting their conservation. Only 3,7% of Chilean bryophytes (55 spp.) have been analyzed using chemical characterization of their extracts (methanol, ether, ethanol, dichloromethane, acetone and hexane). Only four species from Chilean and Argentinian populations (Porella chilensis (Lehm. & Lindenb.) Trevi., Riccardiapolyclada (Mitt. ex Thurn) Hässel,... |
| Tipo: Journal article |
Palavras-chave: Mosses; Liverworts; Hornworts; Uses; Secondary metabolites; Biological activity; In vitro cultivation. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100002 |
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| Gavilan,Natália Helena; Furlan,Fernanda Cardoso; Zorz,Alex Zichner; Oliveira,Leandro Silva de; Campos,Wellington Ferreira; Brondani,Gilvano Ebling. |
| ABSTRACT: Cochlospermum regium roots are used in popular medicine and its extract has diverse phytochemical molecules some with antimicrobial activity, consequently exposing this specie to genetic erosion risks. Thus, the objective of this study was to develop an in vitro multiplication protocol using chemical sterilization of culture medium. Therefore, explants obtained from apical buds of C. regium seedlings were inoculated into with 0.05mg L-1 NAA and 1mg L-1 BAP sterilized by chemical agent sodium hypochlorite (NaOCl) at 0.001%, 0.003% and 0.005% of active chlorine (Cl). Autoclaved culture medium was used as control. Result showed that the contamination by bacterial at 91 days of cultivation was significantly (P<0.05) controlled by autoclaving,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autoclaving; Cerrado biome; Active chlorine; In vitro cultivation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900400 |
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| Grigolo,Chaiane Renata; Citadin,Idemir; Oliveira,Marisa De Cacia; Scariotto,Silvia; Pertille,Rafael Henrique; Santos,Ester Provensi. |
| Abstract Early-ripening peach cultivars present difficulties in the process of natural germination of seeds. In order to solve this problem, the culture of embryos in vitro can be considered an alternative, in which seeds find conditions to complete germination and development in a satisfactory way. Different protocols and culture media have been tested to meet the nutritional needs of the embryo, but they still have problems and need to be optimized. The objective of this work was to test culture media and BAP concentrations in order to increase the germination percentage of embryos and viable ‘BRS Kampai’ peach seedlings, avoiding possible anomalies in the process. The experiment was carried out at the Laboratory of Tissue Culture – “Universidade... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo Cultivation; In vitro cultivation; Cytokine; Prunus persica. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452020000600801 |
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| Mendonça,Evânia Galvão; Paiva,Luciano Vilela; Stein,Vanessa Cristina; Pires,Marinês Ferreira; Santos,Breno Régis; Pereira,Fabricio José. |
| The objective of this work was to elucidate the growth curve of Eucalyptus camaldulensis Dehn. calli analyzing their anatomical modifications. A sigmoid aspect of the growth curve of the calli fresh matter was observed, with five different phases (lag, exponential, linear, deceleration and decline). In the lag phase, the highest growth percentage 87%, was observed, which reduced during the evaluation period to 17% in the linear phase. As for the anatomical analyses, cellular multiplications was observed during the lag and exponential phases and increase in cell size during the linear phase, promoting the calli volume growth and the establishment of the globular conformation. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro cultivation; Organogenesis; Anatomy; Astra blue; Safranin; Forestry. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000600012 |
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| Hirumi, Hiroyuki; Hirumi, K.; Moloo, S. K.; Shaw, M. K.. |
| Bloodstream trypomastigotes (BSFs) of 2 clones (IL 1392 and IL 3671) of Trypanosoma vivax were cultured without feeder layers in 3 systems. System I: metacyclic-producing cultures of T. vivax IL 1392 were initiated with BSFs derived from infected mice at 27 OC in the presence of feeder layers using TVM-1 medium. Metacyclics harvested were then transferred to flasks containing feeder cells and maintained at 34 OC using TVM-22 medium. Under such conditions, the metacyclics transformed to BSFs. Bloodstream trypomastigotes which were then transferred to new flasks, continued to grow in HMI-162 medium without feeder cells. The maximum density of the BSFs and their shortest population doubling time were 3.5 x l06 / ml and 13.5 h, respectively. System II:... |
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Palavras-chave: Feeder cell layers; In vitro cultivation; Trypomastigotes. |
| Ano: 1991 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/153 |
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| Pêgo,Rogério Gomes; Paiva,Patrícia Duarte de Oliveira; Paiva,Renato. |
| The species Syngonanthus elegantulus Ruhland is an important native plant used as cut flowers, but extractive exploration has caused the endangered status of this plant. Therefore, the objective of this study was to establish a protocol of the micropropagation of S. elegantulus. To this end, seed germination was tested in media containing different concentrations of WPM salts. For plantlet establishment, MS and WPM media were evaluated with 50 and 100% of the salt concentrations. Different the sucrose levels in the WPM medium were also evaluated. For multiplication, the plantlets were inoculated in WPM media with 0.0, 0.5, 1.0, 2.0 and 4.0 mg L-1 TDZ and 0.0, 0.5 and 1.0 mg L-1 NAA in all possible combinations. The pre-acclimatization effect was tested in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro cultivation; Floriculture; Conservation; Endangered; Plant. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542013000100004 |
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| Pêgo, Rogério Gomes; Universidade Federal de Viçosa; Paiva, Patrícia Duarte de Oliveira; Universidade Federal de Lavras; Paiva, Renato; Universidade Federal de Lavras. |
| The research aimed to establish a protocol of micropropagation of Syngonanthus elegans. Seed germination in WPM media containing 0%, 25%, 50%, 75% and 100% salt concentrations was evaluated. Establishment of plantlets in WPM and MS media, with 50% or 100% salt concentrations, was studied. The addition of 0, 5, 10, 15, 20, 25 and 30 g L-1of sucrose in WPM media was evaluated. The effects of TDZ (0.0; 0.5; 1.0; 2.0 and 4.0 mg L-1) and ANA (0.0; 0.5 and 1.0 mg L-1) in all possible combinations were investigated. To test acclimatization, sand, PlantmaxTM or vermiculite substrates were evaluated by direct acclimatization and pre-acclimatization. The germination of S. elegans seeds was not influenced by salt concentration in the culture media, but the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.01.00.00-9 floriculture; In vitro cultivation; Multiplication; Acclimatization Agronomia. |
| Ano: 2014 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/17946 |
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| Registros recuperados: 11 | |
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